agosto 14, 2024
Maintaining superior viral vector recovery in cell and gene therapy applications by using Daikyo Crystal Zenith® vials
West partnered with SK Pharmteco to evaluate the impact of vial material of construction on the stability of adeno-associated virus (AAV) serotypes under varying stress and storage conditions associated with cell and gene therapy applications. AAV8 and AAV9 were chosen as representative serotypes, with respective titers in a common buffer formulation (10mM Tris-HCl, 3.5mM NaCl, 8.5% Trehalose, 0.06% PS80, pH 7.2). This was evaluated across three 2mL vial types : Daikyo Crystal Zenith® (CZ), and two commercially available borosilicate glass containers. All containers were sealed with West 13mm Serum NovaPure® 1358 4023/50G bromobutyl stoppers and filled with 0.35mL fill volume.
Vidya Murthy and Eric Kurtz